Publications

Damian M, Pons V, Renault P, M'Kadmi C, Delort B, Hartmann L, Kaya AI, Louet M, Gagne D, Ben Haj Salah K, Denoyelle S, Ferry G, Boutin JA, Wagner R, Fehrentz JA, Martinez J, Marie J, Floquet N, Galès C, Mary S, Hamm HE, Banères JL (2018). GHSR-D2R heteromerization modulates dopamine signaling through an effect on G protein conformation. Proc Natl Acad Sci USA 115:4501-4506.

Hartmann L, Metzger E, Ottelard N, Wagner R (2017). Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris Protoplasts. Methods Mol Biol 1635:45-56.

Chaptal V, Delolme F, Kilburg A, Magnard S, Montigny C, Picard M, Prier C, Monticelli L, Bornert O, Agez M, Ravaud S, Orelle C, Wagner R, Jawhari A, Broutin I, Pebay-Peyroula E, Jault JM, Kaback HR, le Maire M, Falson P (2017). Quantification of Detergents Complexed with Membrane Proteins. Sci Rep 7:41751.

Hartmann L, Kugler V, Wagner R (2016). Expression of Eukaryotic Membrane Proteins in Pichia pastoris. Methods Mol Biol 1432:143-62.

Westh Hansen R, Wang X, Golab A, Bornert O, Oswald C, Wagner R, Martinez KL (2016). Functional Stability of the Human Kappa Opioid Receptor Reconstituted in Nanodiscs Revealed by a Time-Resolved Scintillation Proximity Assay. PLoS One 11(4):e0150658.

Logez C, Damian M, Legros C, Dupré C, Guéry M, Mary S, Wagner R, M'Kadmi C, Nosjean O, Fould B, Marie J, Fehrentz JA, Martinez J, Ferry G, Boutin JA, Banères JL (2016). Detergent-free Isolation of Functional G Protein-Coupled Receptors into Nanometric Lipid Particles. Biochemistry 55(1):38-48.

Logez C, Berger S, Legros C, Banères JL, Cohen W, Delagrange P, Nosjean O, Boutin JA, Ferry G, Simonin F, Wagner R (2014). Recombinant human melatonin receptor MT1 isolated in mixed detergents shows pharmacology similar to that in mammalian cell membranes. PLoS One 9(6):e100616.

Puglia AL, Rezende AG, Jorge SA, Wagner R, Pereira CA, Astray RM (2013). Quantitative RT-PCR for titration of replication-defective recombinant Semliki Forest virus. J Virol Methods 193(2):647-652.

Bornert O, Møller TC, Boeuf J, Candusso MP, Wagner R, Martinez KL, Simonin F (2013). Identification of a novel protein-protein interaction motif mediating interaction of GPCR-Associated Sorting Proteins with G protein-coupled receptors. PLoS One 8(2):e56336.

Logez C, Alkhalfioui F, Byrne B, Wagner R (2012). Preparation of expression plasmids for Pichia pastoris. Methods Mol Biol 866:25-40.

Singh S, Gras A, Fiez-Vandal C, Martinez M, Wagner R, Byrne B (2012). Expression screening in Pichia pastoris. Methods Mol Biol 866:65-73.

Singh S, Gras A, Fiez-Vandal C, Martinez M, Wagner R, Byrne B (2012). Large scale production of membrane proteins in P. pastoris: GPCRs as a case study. Methods Mol Biol 866:197-207.

Bornert O, Alkhalfioui F, Logez C, Wagner R (2012). Overexpression of Membrane Proteins using Pichia pastoris. Curr Protoc Protein Sci Chapter 29:Unit 29.2.

Alkhalfioui F, Logez C, Bornert O, Wagner R (2011). Expression systems: Pichia pastoris. In: Production of Membrane Proteins – Strategies for Expression and Isolation (Robinson A.S., ed.), Wiley-VCH, pp. 75-108.

Brillet K, Pereira CA, Wagner R (2010). Expression of membrane proteins in Drosophila melanogaster S2 cells: production and analysis of a EGFP-fused G protein-coupled receptor as a model. Methods Mol Biol 601:119-33.

Ferndahl C, Bonander N, Logez C, Wagner R, Gustafsson L, Larsson C, Hedfalk K, Darby RAJ, Bill RM (2010). Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory. Microb Cell Fact 9:47.

Michalke K, Huyghe C, Lichière J, Gravière ME, Siponen M, Sciara G, Lepaul I, Wagner R, Magg C, Rudolph R, Cambillau C, Desmyter A (2010). Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1. Anal Biochem 401(1):74-80.

Quinton L, Girard E, Maiga A, Rekik M, Lluel P, Masuyer G, Larregola M, Marquer C, Ciolek J, Magnin T, Wagner R, Molgó J, Thai R, Fruchart-Gaillard C, Mourier G, Chamot-Rooke J, Ménez A, Palea S, Servent D, Gilles N (2010). AdTx1: a natural non-competitive peptide with selective alpha1A-adrenergic antagonist activity. Br J Pharmacol 159(2):316-25.

Alkhalfioui F, Magnin T, Wagner R (2009). From purified GPCRs to drug discovery: the promise of protein-based methodologies. Curr Opin Pharmacol 9(5):629-35.

Benmaamar R, Astray RM, Wagner R, Pereira CA (2009). High-level expression of rabies virus glycoprotein with the RNA-based Semliki Forest Virus expression vector. J Biotechnol 139:283-90.

Magnin T, Fiez-Vandal C, Potier N, Coquard A, Leray I, Steffan T, Logez C, Alkhalfioui F, Pattus F, Wagner R (2009). A novel, generic and effective method for the rapid purification of G protein-coupled receptors. Prot Expr Purif 64:1-7.

Michalke K, Gravière ME, Huyghe C, Vincentelli R, Wagner R, Pattus F, Schroeder K, Oschmann J, Rudolph R, Cambillau C, Desmyter A (2009). Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies. Anal Biochem 386:147-55.

Brillet K, Perret B, Klein V, Pattus F, Wagner R (2008). Using EGFP fusions to monitor the functional expression of GPCRs in the Drosophila Schneider 2 cells. Cytotechnology 57:101-109.

Singh S, Gras A, Fiez-Vandal C, Ruprecht J, Rana R, Martinez M, Strange P, Wagner R, Byrne B (2008). Large-scale functional expression of WT and truncated human Adenosine A2A Receptor in Pichia pastoris bioreactor cultures. Microb Cell Fact 7:28.

André N, Cherouati N, Prual C, Steffan T, Zeder-Lutz G, Magnin T, Pattus F, Michel H, Wagner R, Reinhart C (2006). Enhancing functional production of G Protein-Coupled Receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci 15(5):1115-26.

Hassaine G, Wagner R, Kempf J, Cherouati N, Hassaine N, Prual C, André N, Reinhart C, Pattus F, Lundstrom K (2006). Semliki Forest Virus vectors for overexpression of 101 G protein-coupled receptors in mammalian host cells. Prot Expr Purif 45(2):343-51.

Lundstrom K, Wagner R, Reinhart C, Desmyter A, Cherouati N, Magnin T, Zeder-Lutz G, Courtot M, Prual C, André N, Hassaine G, Michel H, Cambillau C, Pattus F (2006). Structural genomics on membrane proteins - Comparison of more than 100 GPCRs in 3 expression systems. J Struct Funct Genomics 7(2):77-91.

Zeder-Lutz G, Cherouati N, Reinhart C, Pattus F, Wagner R (2006). Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris. Prot Expr Purif 50(1):118-27.

Perret B, Wagner R, Lecat S, Rabut G, Brillet K, Bucher B, Pattus F (2003). Expression of EGFP-amino-tagged human mu opioid receptor in Drosophila Schneider 2 cells: a potential expression system for large-scale production of G-protein coupled receptors. Prot Expr Purif 31:123-132.